THE HYDROLYSIS OF PENICILLIN G TO 6-AMINO PENICILLANIC ACID BY ENTRAPPED PENICILLIN ACYLASE
نویسندگان
چکیده
منابع مشابه
Continuous Production of 6-amino Penicillanic Acid (6-APA) by Agarose Immobilized Penicillin Acylase in a Packed Column Reactor
Penicillin acylase, an industrially important biocatalyst catalyzes the conversion of penicillins to 6-amino penicillanic acid (6-APA) which is the main precursor for the production of semi-synthetic -lactam antibiotics. The present work involves the continuous production of 6-APA in a packed column reactor by using agarose immobilized penicillin acylase as a block polymer. The strain Escherich...
متن کاملKinetic Parameter Studies of 6-Amino Penicillanic Acid (6-APA) Production by Agarose Immobilized Penicillin Acylase in a Packed Column Reactor
Penicillin acylase, an industrially important biocatalyst catalyzes the conversion of penicillins to 6-amino penicillanic acid (6-APA) which is the main precursor for the production of semisynthetic ß-lactam antibiotics. The present work involves the continuous production of 6APA in a packed column reactor by using agarose immobilized penicillin acylase as a block polymer. The strain Escherichi...
متن کاملProduction of Penicillin Acylase.
The production of penicillin acylase by Escherichia coli Ny.I/3-67 has been increased by phenylacetic acid and phenoxyacetic acid, which themselves strongly inhibit the function of this specific enzyme. Other carbonic acids also increased penicillin acylase production, but to a lesser degree; they also weakly inhibited enzyme function. The production of this enzyme was effectively repressed wit...
متن کاملOptimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase
Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...
متن کاملOptimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase
Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1973
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.26.228